Dynamics of T helper subpopulations in the critical period of severe injury in children

Severe mechanical injury is one of the main reasons behind children’s disability and mortality. induces a complex host immune response to tissue injury, parallel pro- anti-inflammatory state, bearing an elevated risk for infectious complications (IC) and/or multiple organ failure (MOF). This study aimed determine informative immunological criteria traumatic severity prognosis outcome in children (severe group (SInj, ISS ≥ 16), n = 87; mild/moderate (MInj, < 34) based on assessment absolute cell count (abs) percentage such T helper subpopulations as regulatory lymphocytes – CD4 + CD127 low CD25 high (Treg), Th17 CD161 higt cells(T127hi) severe cases grouped by (favorable, 47; unfavorable, 40) depending IC (n 16) development MOF 11) 1 st , 3 d 5 th 7 14 day after injury. The control was comprised 80 apparently healthy comparable age sex. An inverse relationship between degree blood loss revealed with abs all Th populations, but Treg most significant correlation found (Spearman’s R ≤ -0,70, p 0.00001). For SInj group, pronounced decrease abs, T127hi acute post-traumatic period increase days revealed. values first indicators patients MInj correspond significantly differ from group. There are different kinetics peripheral injuries. Th17%CD4 T127hi%CD4 -3 -7 respectively comparation сontrol groups. were no differences groups terms Treg%CD4 . lower-level trauma admitted ICU associated develop trauma. reduced 3-7 MOF. results indicate that levels Treg, may be used predict assess